Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5030774 | Biosensors and Bioelectronics | 2018 | 26 Pages |
Abstract
A novel, label-free and convenient strategy for thrombin assay has been developed based on the fluorescence resonance energy transfer (FRET) from a cationic conjugated polymer (CCP) to Ir(III) complex. The energy donor (CCP) and acceptor (Ir(III) complex) were taken into close proximity through Ï-Ï stacking interaction and electrostatic interaction, leading to the occurrence of FRET. However, the introduction of the thrombin aptamer upset the status and blocked the FRET process, but afterwards the reappearance of FRET phenomenon was confirmed by the special binding interaction between aptamer and thrombin, thus achieving the quantitative detection of thrombin. This assay could detect thrombin as low concentration as about 0.05Â pM and provided a highly specific selectivity among other nonspecific proteins. Moreover, the strategy may allow our platform to provide similar sensitivity toward different targets in an aptamer-structure-independent manner. Furthermore, the assay can be used to detect thrombin in diluted real urine or serum samples with a satisfactory recovery, implying its great potential for rapid detection of thrombin in the clinic.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Chunnuan Du, Yufang Hu, Qingqing Zhang, Zhiyong Guo, Guoping Ge, Sui Wang, Chunyang Zhai, Mingshan Zhu,