Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5030866 | Biosensors and Bioelectronics | 2017 | 7 Pages |
â¢A simple to fabricate low cost Thin Film Transistor sensor.â¢Ultra fast isothermal DNA amplification and detection.â¢DNA amplification measured by pH change.â¢Detected genes coding for antimicrobial resistance.
A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremely fast and sensitive detection. It can detect <10 copies of the gene in genomic DNA extracted from E. coli or K. pneumoniae clinical isolates within a few minutes. A differential readout system was developed to minimize the effect of primer-dimer amplification on the assay. The simple device has the potential for low cost, portable and real-time nucleic acid analysis as a Point of Care device.