Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5031084 | Biosensors and Bioelectronics | 2017 | 6 Pages |
Abstract
In the present work, electrogenerated chemiluminescence (ECL) of luminol was investigated in neutral condition at a gold electrode in the presence of silicon quantum dots (SiQDs). The results revealed that SiQDs can not only greatly enhance luminol ECL, but also act as energy acceptor to construct a novel ECL resonance energy transfer (ECL-RET) system with luminol. As a result, strong anodic ECL signal was obtained in neutral condition at the bare gold electrode, which is suitable for biosensing application. Lysozyme exhibited apparent inhibiting effect on the ECL-RET system, based on which an ECL aptasensor was fabricated for the sensitive detection of lysozyme. The proposed method showed high sensitivity, good selectivity, and wide linearity for the detection of lysozyme in the range of 5.0Ã10-14â5.0Ã10â9 g mLâ1 with a detection limit of 5.8Ã10-15 g mLâ1 (3Ï). The results suggested that as-proposed luminol/SiQDs ECL biosensor will be promising in the detection enzyme.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yong-Ping Dong, Jiao Wang, Ying Peng, Jun-Jie Zhu,