Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5031108 | Biosensors and Bioelectronics | 2017 | 4 Pages |
Abstract
A virulent phage named as PaP1 was isolated from hospital sewage based on a lambda phage isolation protocol. This phage showed a strong and highly specific binding ability to Pseudomonas aeruginosa (P. aeruginosa). Using this isolated phage as a recognition agent, a novel electrochemiluminescent (ECL) biosensor was developed for label-free detection of P. aeruginosa. The biosensor was fabricated through depositing phage-conjugated carboxyl graphene onto the surface of a glass carbon electrode. After specific binding of the host bacteria through the adsorption of P. aeruginosa cell wall by phage tail fibers and baseplate, the ECL signal of luminol suffered a decrease since the formed non-conductive biocomplex obstructed the interfacial electron transfer and blocked the diffusion of the ECL active molecules. The ECL emission declined linearly with P. aeruginosa concentration in the range of 1.4Ã102 â1.4Ã106Â CFUÂ mLâ1, with a very low detection limit of 56Â CFUÂ mLâ1. The whole detection process could be completed within 30Â min as a ready-for-use biosensor was adopted. This biosensor was successfully applied to quantitate P. aeruginosa in milk, glucose injection and human urine with acceptable recovery values ranging from 78.6% to 114.3%.
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Authors
Huan Yue, Yong He, Enci Fan, Lin Wang, Shuguang Lu, Zhifeng Fu,