Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5031137 | Biosensors and Bioelectronics | 2017 | 21 Pages |
Abstract
We present a non-modification electrochemical DNA sensing strategy, which used Potential-Assisted Au-S Deposition and a clamp-like DNA probe. The dual-hairpin probe DNA was tagged with a methylene blue (MB) at the 3â² terminal and a thiol at the 5â² terminal., Without being hybridized with target DNA, the loop of probe prevented the thiol from reaching the bare gold electrode surface with an applied potential., After hybridization with the target DNA, the probe' s loop-stem structure opened through two distinct and sequential events, which led to the formation of a triplex DNA structure. Then the thiol easily contacted with electrode and resulted in potential-assisted Au-S self-assembly. Electrochemical signals of MB were measured by differential pulse voltammetry (DPV) and used for target quantitative detection. This strategy offered a detection limit down to 2.3Â pM. and an inherently high specificity for detecting even single mismatch.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nian Hong, Lin Cheng, BingGuo Wei, ChaDan Chen, Ling Ling He, DeRong kong, JinXiang Ceng, Han-Feng Cui, Hao Fan,