Article ID Journal Published Year Pages File Type
5031158 Biosensors and Bioelectronics 2017 6 Pages PDF
Abstract
In this paper, we demonstrated that kanamycin could enhance the peroxidase-like activity of citrate-capped gold nanoparticles (AuNPs) through two steps: the attachment of kanamycin onto AuNPs through −NH2 (on kanamycin) and −COOH (on AuNPs) interactions; and the specifically interaction between glucoside on kanamycin and AuNPs which changes the surface property of AuNPs, and produced
- OH radicals and Au3+ in the solution, and catalyzed the chromogenic reactions between 3, 3′, 5, 5′-tetramethylbenzidine (TMB) and H2O2. Based on this principle, a novel method for kanamycin detection has been developed. This method exhibited high sensitivity and selectivity, as low as 0.1 nM kanamycin could be detected with a linear range from 0.1 nM to 20 nM and 20nM to 300nM, respectively. This method was also successfully applied for the detection of kanamycin content in milk and meat samples.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , , ,