Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5031220 | Biosensors and Bioelectronics | 2017 | 32 Pages |
Abstract
The amplification of the target nucleic acid sequence represents a key step for the development of sensitive detection protocols. The integration in microfluidic devices of the most popular technology for nucleic acids amplifications, polymerase chain reaction (PCR), is significantly limited by the thermal cycling needed to obtain the target sequence amplification. This review provides an overview of recent advances in integration of isothermal amplification methods in microfluidic devices. Isothermal methods, that operate at constant temperature, have emerged as promising alternative to PCR and greatly simplify the implementation of amplification methods in point-of-care diagnostic devices and devices to be used in resource-limited settings. Possibilities offered by isothermal methods for digital droplet amplification are discussed.
Keywords
ddPCRSingle-strand DNA binding proteinSSBRPART-LAMPssDNAHCRHDARCASDAMicrofluidicsdsDNASingle-stranded DNAdouble-stranded DNADNAIsothermal amplificationLoop-mediated isothermal amplificationStrand displacement amplificationRolling circle amplificationRecombinase polymerase amplificationnucleic acid sequence-based amplificationNASBADroplet digital PCRLAMPMicroRNAHybridization chain reactionPCR
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Maria Chiara Giuffrida, Giuseppe Spoto,