Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5031224 | Biosensors and Bioelectronics | 2017 | 52 Pages |
Abstract
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and forensic identification. In the past three decades, PCR has evolved from end-point PCR, through real-time PCR, to its current version, which is the absolute quantitive digital PCR (dPCR). In this review, we first discuss the principles of all key steps of dPCR, i.e., sample dispersion, amplification, and quantification, covering commercialized apparatuses and other devices still under lab development. We highlight the advantages and disadvantages of different technologies based on these steps, and discuss the emerging biomedical applications of dPCR. Finally, we provide a glimpse of the existing challenges and future perspectives for dPCR.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lei Cao, Xingye Cui, Jie Hu, Zedong Li, Jane Ru Choi, Qingzhen Yang, Min Lin, Ying Hui Li, Feng Xu,