Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5031398 | Biosensors and Bioelectronics | 2017 | 7 Pages |
Abstract
In this work, tyrosine-protected gold nanoparticles (Tyr-Au NPs) were fabricated by one-step reduction of Au3+ ion using Tyr as a reducing and capping agent under microwave irradiation. The Tyr-Au NPs were successfully used as a dual probe for colorimetric and fluorescence turn-on assays of spermine and spermidine in biological samples. Upon addition of spermine and spermidine, the characteristic surface plasmon resonance (SPR) band of Tyr-Au NPs was red-shifted to 596 and 616Â nm and the emission peak (Tyr) at 410Â nm was gradually increased with increasing concentration of both analytes, confirming the aggregation of Tyr-Au NPs induced by spermine and spermidine, which results to restore fluorescence of Tyr on the surfaces of Au NPs. In addition, it shows high selectivity for sensitive detection of prostatic cancer biomarkers spermine and spermidine in real clinical applications with reduced sample preparations.
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Karuna A. Rawat, Jigna R. Bhamore, Rakesh Kumar Singhal, Suresh Kumar Kailasa,