Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5031402 | Biosensors and Bioelectronics | 2017 | 20 Pages |
Abstract
Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4 °C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
A. Ben Aissa, A. Herrera-Chacon, R.R. Pupin, M.D.P.T. Sotomayor, M.I. Pividori,