Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5031470 | Biosensors and Bioelectronics | 2017 | 23 Pages |
Abstract
In this work, electrochemiluminescence (ECL) luminophor Ru(bpy)32+ was encapsulated in 3D CuNi oxalate and the synthesized metal-inorganic frameworks {[Ru(bpy)3][Cu2xNi2(1âx)(ox)3]}n (Ru/Cu/Ni) exhibited excellent and stable ECL signals, which could be decreased by polyethylenimine capped graphene oxide (GO-PEI). Based on this, a new sandwich ECL immunosensor was developed for detection of carcinoembryonic antigen (CEA). To capture primary antibody and second antibody more simply and efficiently, Ag nanoparticles were doped with Ru/Cu/Ni and GO-PEI. After a sandwich-type immunoreaction, a remarkable decrease of ECL signal was observed due to the release of Ru(bpy)32+ which was caused by the coordination between PEI and metal ions. Under the optimization of determination conditions, a linear response range for CEA from 0.1 pg mLâ1 to 100 ng mLâ1 was obtained, and the detection limit was calculated to be 0.027 pg mLâ1 (S/N=3). The prepared CEA immunosensor displayed high sensitivity, excellent stability and good specificity.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiaojian Li, Siqi Yu, Tao Yan, Yong Zhang, Bin Du, Dan Wu, Qin Wei,