| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5031558 | Biosensors and Bioelectronics | 2017 | 7 Pages |
Abstract
The device has been designed, fabricated and tested for the implementation of DNA amplification through the Polymerase Chain Reaction (PCR) with thermal cycling among three different temperatures on a single site. The glass has been connected to an electronic system that drives the heaters and controls the temperature and light sensors. It has been optically and thermally coupled with another glass hosting a microfluidic network made in polydimethylsiloxane that includes thermally actuated microvalves and a PCR process chamber. The successful DNA amplification has been verified off-chip by using a standard fluorometer.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
G. Petrucci, D. Caputo, N. Lovecchio, F. Costantini, I. Legnini, I. Bozzoni, A. Nascetti, G. de Cesare,