Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5032105 | Journal of Biomechanics | 2017 | 8 Pages |
Abstract
A potent regulator of bone anabolism is physical loading. However, it is currently unclear whether physical stimuli such as fluid shear within the marrow cavity is sufficient to directly drive the osteogenic lineage commitment of resident mesenchymal stem cells (MSC). Therefore, the objective of the study is to employ a systematic analysis of oscillatory fluid flow (OFF) parameters predicted to occur in vivo on early MSC osteogenic responses and late stage lineage commitment. MSCs were exposed to OFF of 1Â Pa, 2Â Pa and 5Â Pa magnitudes at frequencies of 0.5Â Hz, 1Â Hz and 2Â Hz for 1Â h, 2Â h and 4Â h of stimulation. Our findings demonstrate that OFF elicits a positive osteogenic response in MSCs in a shear stress magnitude, frequency, and duration dependent manner that is gene specific. Based on the mRNA expression of osteogenic markers Cox2, Runx2 and Opn after short-term fluid flow stimulation, we identified that a regime of 2Â Pa shear magnitude and 2Â Hz frequency induces the most robust and reliable upregulation in osteogenic gene expression. Furthermore, long-term mechanical stimulation utilising this regime, elicits a significant increase in collagen and mineral deposition when compared to static control demonstrating that mechanical stimuli predicted within the marrow is sufficient to directly drive osteogenesis.
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Authors
Elena Stavenschi, Marie-Noelle Labour, David A. Hoey,