Review of the fluid flow within intervertebral discs - How could in vitro measurements replicate in vivo?

Analyses of discrepancies in the flow-dependent recoveries in vivo and in vitro highlight an excessive fluid content in the latter as a likely cause. To replicate in vivo conditions as closely as possible in vitro, preparation and preconditioning of specimens and/or pressure and osmolarity of the culture media in which specimens are immersed should hence be designed in a manner as to diminish disc hydration level and/or fluid transport.