Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5032585 | Medical Engineering & Physics | 2017 | 11 Pages |
Abstract
Cellular transplantation may treat several human diseases by replacing damaged cells and/or providing a local source of trophic factors promoting regeneration. We utilized human renal epithelial cells (hRECs) isolated from cadaveric donors as a cell model. For efficacious implementation of hRECs for treatment of kidney diseases, we evaluated a novel encapsulation strategy for immunoisolation of hRECs and lentiviral transduction of the Green Fluorescent Protein (GFP) as model gene for genetic engineering of hRECs to secrete desired trophic factors. In specific, we determined whether encapsulation through conformal coating and/or GFP transduction of hRECs allowed preservation of cell viability and of their trophic factor secretion. To that end, we optimized cultures of hRECs and showed that aggregation in three-dimensional spheroids significantly preserved cell viability, proliferation, and trophic factor secretion. We also showed that both wild type and GFP-engineered hRECs could be efficiently encapsulated within conformal hydrogel coatings through our fluid dynamic platform and that this resulted in further improvement of cell viability and trophic factors secretion. Our findings may lay the groundwork for future therapeutics based on transplantation of genetically engineered human primary cells for treatment of diseases affecting kidneys and potentially other tissues.
Keywords
RPMALGMCP-1MMP-2HBSSDTTTGFαGFPCCEGM-CSFSCFDVSPAI-1VCAM-1Three-dimensionalHGFPPGpHEMAPBS3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromideEGFHRECsMOIMTTAlginateCell encapsulationtransforming growth factor alphatriethanolaminetwo-dimensionalDivinyl SulfonedithiothreitolTIMPrevolutions per minuteepidermal growth factorHepatocyte growth factorVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)basic fibroblast growth factorStem Cell FactorTrophic factorsThree-dimensional culturematrix metalloproteinases-2granulocyte macrophage colony-stimulating factorPhosphate-buffered salineHanks' balanced salt solutionPlasminogen activator inhibitor-1Tissue inhibitor of metalloproteinasevascular cell adhesion molecule-1Hydrogelgreen fluorescent proteinpoly(2-hydroxyethyl methacrylate)polyethylene glycolPolypropylene glycolPEGConformal coatingTEAmultiplicity of infectionGene therapy
Related Topics
Physical Sciences and Engineering
Engineering
Biomedical Engineering
Authors
Vita Manzoli, David C. Colter, Sridevi Dhanaraj, Alessia Fornoni, Camillo Ricordi, Antonello Pileggi, Alice A. Tomei,