Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5041031 | Brain, Behavior, and Immunity | 2016 | 11 Pages |
â¢Glucocorticoids (GCs) regulate lymphocyte development and survival.â¢Lymphoid organs can produce GCs via de novo synthesis or regeneration of metabolites.â¢Bone marrow, spleen, and thymus from neonates and adults convert metabolites into GCs.â¢Incubation with the GC metabolite DHC causes 11β-HSD1- and GR-dependent apoptosis.â¢GC regulation of lymphoid development and function may be controlled by 11β-HSD1.
Glucocorticoids (GCs) are circulating adrenal steroid hormones that coordinate physiology, especially the counter-regulatory response to stressors. While systemic GCs are often considered immunosuppressive, GCs in the thymus play a critical role in antigen-specific immunity by ensuring the selection of competent T cells. Elevated thymus-specific GC levels are thought to occur by local synthesis, but the mechanism of such tissue-specific GC production remains unknown. Here, we found metyrapone-blockable GC production in neonatal and adult bone marrow, spleen, and thymus of C57BL/6 mice. This production was primarily via regeneration of adrenal metabolites, rather than de novo synthesis from cholesterol, as we found high levels of gene expression and activity of the GC-regenerating enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), but not the GC-synthetic enzyme CYP11B1. Furthermore, incubation with physiological concentrations of GC metabolites (11-dehydrocorticosterone, prednisone) induced 11β-HSD1- and GC receptor-dependent apoptosis (caspase activation) in both T and B cells, showing the functional relevance of local GC regeneration in lymphocyte GC signaling. Local GC production in bone marrow and spleen raises the possibility that GCs play a key role in B cell selection similar to their role in T cell selection. Our results also indicate that local GC production may amplify changes in adrenal GC signaling, rather than buffering against such changes, in the immune system.