| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 50495 | Catalysis Communications | 2013 | 5 Pages |
Abstract
•Candida antarctica lipase B was subjected to directed evolution by CAST and ISM.•Mutants showed increased activity and selectivity toward three profen esters.•High enantioselectivity for flurbiprofen and ketoprofen esters was obtained.
The lipase B from Candida antarctica was subjected to directed evolution suggested by its structure. Mutants of the lipase show significantly increased activity and enantioselectivity toward profen esters compared to the wild type, especially for flurbiprofen ester (ee = 93%, E = 37) and ketoprofen ester (ee = 99%, E > 200).
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Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Bin Qin, Ping Liang, Xian Jia, Xin Zhang, Mao Mu, Xiao-Ying Wang, Guo-Zhen Ma, Dan-Ni Jin, Song You,