Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
50869 | Catalysis Communications | 2010 | 4 Pages |
Abstract
Polyethyleneimine (PEI) modification on immobilized lipases greatly enhanced their enantioselectivity in the kinetic resolution of (±)-2-hydroxy-phenylacetic acid methyl ester. The enantiomeric ratio (E) of CNBr-agarose-CRL rose from 8 (without coating) to 20 (ee = 90%) after PEI coating in the hydrolysis at pH 5. In the case of CAL-B, the coating highly improved the enantioselectivity of the immobilized lipase from E = 1.5 (without coating) to E > 100 (ee > 99%). Moreover, this coating strategy improved the stability of the biocatalyst at high temperatures and in the presence of high co-solvent concentrations.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Zaida Cabrera, Melissa L.E. Gutarra, Jose M. Guisan, Jose M. Palomo,