Studies of Fe3O4-chitosan nanoparticles prepared by co-precipitation under the magnetic field for lipase immobilization

A simple method was introduced to prepare magnetic Fe3O4-chitosan nanoparticles by co-precipitation with 0.45 T static magnetic field via glutaraldehyde cross-linking reaction, and the nanoparticles were used to immobilize lipase. The influence of magnetic field on the properties of magnetic nanoparticles was studied by SEM, XRD and VSM. The results showed that no obvious difference of the nanoparticles structure was found, while the morphology varied from random sphere-like to rod-like and magnetic character was significantly changed. Furthermore, the lipase immobilized with nanoparticles prepared under the magnetic field enhanced the activity, operational and storage stability compared with the control sample.

Graphical AbstractSEM images of Fe3O4-CS nanoparticles prepared without (a) and with (b) MF.Figure optionsDownload full-size imageDownload as PowerPoint slideResearch Highlights► We prepared Fe3O4-chitosan nanoparticles by co-precipitation method under the MF. ► We studied the influence of the MF on the properties of magnetic nanoparticles. ► Not like the crystal structure, the morphology and magnetic character were changed. ► The lipase was immobilized with nanoparticles prepared under the MF. ► Activity and stability of the immobilized lipase were improved.