Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5131457 | Analytical Biochemistry | 2017 | 4 Pages |
Abstract
Cyanine 5 (Cy5) is an established fluorescent dye in microarray analysis. It is degraded rapidly when exposed to atmospheric ozone during post-hybridization washes, which leads to loss of fluorescent intensity. To minimize this undesirable effect, we coated microarray slides with sodium dodecyl sulfate (SDS) solution at post-hybridization washes. The fluorescent intensities on coated slides were more stable than those on uncoated slide. We also performed the microarrays with SDS solution for a year to check the solution's effectiveness along with seasonal changes of atmospheric ozone level. Consistent results in microarray analysis were obtained using Cy5 dye under atmospheric ozone.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Youngjun Kim, Hyun Hee Seo, Mi Seon Jeong, Ki Heon Lee, In Ho Lee, Kyeong A. So, Mi Kyung Kim, Yoo-Kyung Lee, Seon Ah Kim, Tae Jin Kim,