Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5131535 | Analytical Biochemistry | 2017 | 5 Pages |
Abstract
Biotin-4-fluorescein (B4F) is a commonly used fluorescent probe for studying biotin-(strept)avidin interactions. During a characterization study of an anti-biotin antibody, using B4F as the probe, we noticed a discrepancy in the expected and experimentally determined number of biotin binding sites. Analytical testing showed that the biotin moiety in the probe undergoes a photosensitized oxidation to produce a mixture of biotin sulfoxides which has the potential to impact the quantitation of binding sites using this fluorescent probe.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Richard A. Haack, Kerry M. Swift, Qiaoqiao Ruan, Richard J. Himmelsbach, Sergey Y. Tetin,