| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5131551 | Analytical Biochemistry | 2017 | 5 Pages |
Abstract
A rapid, convenient and efficient DNA extraction method with no need for toxic agents and centrifugation was reported. A polypropylene microfuge (MF) tube was used as the substrate to immobilize polydopamine (PDA). The prepared PDA-coated MF (PDA@MF) tube was used as a pH-responsive platform for rapid extraction of DNA based on pH-induced charge switch of amino and phenolic hydroxyl groups in PDA coating. The extraction procedure is simple and can be finished in 25Â min. The PDA@MF tube was applied for extraction of genomic DNA from foodborne pathogens in milk. The extracted DNA was directly used as template for PCR amplification.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Zitao Zhong, Xin Yao, Xiaomei Gao, Li Jia,