| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5131565 | Analytical Biochemistry | 2017 | 4 Pages |
Abstract
Poly(ADP-ribose) polymerases (PARPs) have been implicated in responses of plants to DNA damage and numerous stresses, whereby the mechanistic basis of the interference is often unclear. Therefore, the identification of specific inhibitors and potential interactors of plant PARPs is desirable. For this purpose, we established an assay based on heterologous expression of PARP genes from the model plant Arabidopsis thaliana in yeast. Expression of AtPARPs caused an inhibition of yeast growth to different extent, which was alleviated by inhibitors targeted at human PARPs. This assay provides a fast and simple means to identify target proteins and pharmacological inhibitors of AtPARP1.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Dagmar Rissel, Peter Paul Heym, Edgar Peiter,