| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5131674 | Analytical Biochemistry | 2017 | 4 Pages |
Abstract
Botulinum neurotoxins (BoNTs) are the most toxic proteins in nature. Endopeptidase-mass-spectrometry (Endopep-MS) is used as a specific and rapid in-vitro assay to detect BoNTs. In this assay, immunocaptured toxin cleaves a serotype-specific-peptide-substrate, and the cleavage products are then detected by MS. Here we describe the design of a new peptide substrate for improved detection of BoNT type A (BoNT/A). Our strategy was based on reported BoNT/A-SNAP-25 interactions integrated with analysis method efficiency considerations. Integration of the newly designed substrate led to a 10-fold increase in the assay sensitivity both in buffer and in clinically relevant samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Osnat Rosen, Liron Feldberg, Eyal Dor, Sigalit Gura, Ran Zichel,