Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5131753 | Analytical Biochemistry | 2017 | 4 Pages |
Abstract
Calibration-free concentration analysis (CFCA) based on surface plasmon resonance uses the diffusion coefficient of an analyte to determine the concentration of that analyte in a bulk solution. In general, CFCA is avoided when investigating analytes prone to self-association, as the heterogeneous diffusion coefficient results in a loss of precision. The derivation for self-association of the analyte was presented here. By using the diffusion coefficient for the monomeric state, CFCA provides the lowest possible concentration even though the analyte is self-associated.
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Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hiroshi Imamura, Shinya Honda,