Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5131806 | Analytical Biochemistry | 2017 | 5 Pages |
Abstract
A ring-shaped electroeluter (RSE) was designed for protein recovery from polyacrylamide gel matrix. The RSE was designed in such a way that a ring-shaped well was used to place gel slices and an enrichment well was used to collect eluted protein samples. With HSA as model protein, the electroelution time was less than 30Â min with 80% recovery rate, and the concentration of recovered protein was 50 times higher than that of conventional method. The RSE could be reused at least ten times. The developed device makes great advance towards economic electroelution of biomolecules (such as proteins) from gel matrix.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jing-Jing Chen, Guo-Qing Li, Amit Pratush, Sharmin Jahan, Fan-Zhi Kong, Hua Xiao, Liu-Yin Fan, Cheng-Xi Cao,