Advances in MALDI imaging mass spectrometry of proteins in cardiac tissue, including the heart valve

•Protein imaging of cardiac tissue is underrepresented in MALDI IMS studies.•We discuss and demonstrate current MALDI IMS approaches that may be used to detail proteins from cardiac tissue.•MALDI IMS has the potential to become an defining tool for proteomic studies in cardiovascular research/

Significant progress has been made for tissue imaging of proteins using matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS). These advancements now facilitate mapping of a wide range of proteins, peptides, and post-translational modifications in a wide variety of tissues; however, the use of MALDI IMS to detect proteins from cardiac tissue is limited. This review discusses the most recent advances in protein imaging and demonstrates application to cardiac tissue, including the heart valve. Protein imaging by MALDI IMS allows multiplexed histological mapping of proteins and protein components that are inaccessible by antibodies and should be considered an important tool for basic and clinical cardiovascular research. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann.