Analytical MethodsStraightforward analytical method to determine opium alkaloids in poppy seeds and bakery products

•Straightforward method by LC-MS/MS to determine opium alkaloids in poppy seeds.•The scope of the method covers morphine, codeine, thebaine, noscapine, papaverine and narceine.•Morphine, codeine and thebaine were detected in 100%, 78% and 78% of the samples.•60% of the samples exceed the German guidance value of 4 mg/kg of morphine set in Germany.

A straightforward method to determine the content of six opium alkaloids (morphine, codeine, thebaine, noscapine, papaverine and narceine) in poppy seeds and bakery products was developed and validated down to a limit of quantification (LOQ) of 0.1 mg/kg. The method was based on extraction with acetonitrile/water/formic acid, ten-fold dilution and analysis by LC-MS/MS using a pH 10 carbonate buffer.The method was applied for the analysis of 41 samples collected in 2015 in the Netherlands and Germany. All samples contained morphine ranging from 0.2 to 240 mg/kg. The levels of codeine and thebaine ranged from below LOQ to 348 mg/kg and from below LOQ to 106 mg/kg, respectively. Sixty percent of the samples exceeded the guidance reference value of 4 mg/kg of morphine set by BfR in Germany, whereas 25% of the samples did not comply with the limits set for morphine, codeine, thebaine and noscapine by Hungarian legislation.