Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5133102 | Food Chemistry | 2018 | 6 Pages |
â¢Thirteen phenolic compounds were isolated from Pyrus ussuriensis Maxim..â¢Ten compounds were obtained from Pyrus ussuriensis Maxim. for the first time.â¢The antioxidant activities of these isolated phenolic compounds were determined.â¢Among the isolated compounds, quercetin presented the highest antioxidant activity.
Thirteen phenolic compounds were isolated from pear (Pyrus ussuriensis Maxim.) peels and leaves extracts by using various column chromatography techniques with a guided DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging assay, the result of antioxidant activity of phenolic compounds is then verified by measurement of ROS (reactive oxygen species). The isolated compounds were identified as rutin (1), (â)-catechin (2), orobol (3), daidzein (4), tricin 4â²-O-[threo-β-guaiacyl-(7â³-O-methyl)-glyceryl] ether (5), tricin 4â²-O-[threo-β-guaiacyl-(7â³-O-methyl-9â³-O-acetyl)-glyceryl] ether (6), 5,7,3â²,5â²-tetrahydroxyflavanone (7), artselaeroside A (8), trilobatin (9), 3-(2,4,6-trihydroxyphenyl)-1-(4-hydroxyphenyl)-propan-1-one (10), quercetin-3-O-(3â³-O-galloyl)-α-l-rhamnopyranoside (11), apigenin (12) and quercetin (13) on the basis of nuclear magnetic resonance spectroscopy along with comparison with literature data. Among these compounds, quercetin and quercetin-3-O-(3â³-O-galloyl)-α-l-rhamnopyranoside exhibited potent DPPH radical-scavenging activity with IC50 (Half Maximal Inhibitory Concentration) value of 6.06 and 9.60 μg/mL, respectively. The results revealed that P. ussuriensis could be used in the fields of food and medicine to prevent human aging diseases.