Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5133183 | Food Chemistry | 2017 | 9 Pages |
â¢DNA barcoding was combined with High Resolution Melting (HRM) analysis.â¢COI and cytb barcode regions were exploited for Gadidae species differentiation.â¢Atlantic cod, Pacific cod, pollock and saithe were identified successfully.â¢Cytb was the most efficient barcode to differentiate the four species by HRM analysis.â¢A new, rapid, simple and cost-effective tool was proposed for codfish authentication.
This work aimed to exploit the use of DNA mini-barcodes combined with high resolution melting (HRM) for the authentication of gadoid species: Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Theragra chalcogramma) and saithe (Pollachius virens). Two DNA barcode regions, namely cytochrome c oxidase subunit I (COI) and cytochrome b (cytb), were analysed in silico to identify genetic variability among the four species and used, subsequently, to develop a real-time PCR method coupled with HRM analysis. The cytb mini-barcode enabled best discrimination of the target species with a high level of confidence (99.3%). The approach was applied successfully to identify gadoid species in 30 fish-containing foods, 30% of which were not as declared on the label. Herein, a novel approach for rapid, simple and cost-effective discrimination/clustering, as a tool to authenticate Gadidae fish species, according to their genetic relationship, is proposed.