Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5133887 | Food Chemistry | 2017 | 7 Pages |
â¢A LC/MS/MS method for analysis of isoeugenol in finfish is presented.â¢A rapid and environmentally friendly extraction method was developed for isoeugenol in finfish.â¢Application of a dansylation derivatization technique significantly increases the sensitivity of the method.
A simple, rapid, selective and sensitive LC-MS/MS method was developed and validated for the determination of isoeugenol in finfish using an off-line dansylation derivatization step to enhance signal intensity. The sample was extracted with 10 mL acetone using a tissue homogenizer, followed by derivatization with dansyl chloride and analysis by liquid chromatography tandem mass spectrometry. The method detection limit ranged from 0.2 to 0.7 ng gâ1 in six fish species. The limit of quantification was 2.5 ng gâ1 in each species. Recoveries of isoeugenol at three different levels (5, 10 and 20 ng gâ1) over two different days ranged from 91.2% to 108.0%. Precision (%RSDR) values determined at 10 ng gâ1 were 4.3%, 8.0%, 5.3%, 5.1%, 2.6% and 8.0% for tilapia, catfish, trout, salmon, hybrid striped bass and yellow perch, respectively. Studies of linearity in the range 2.5-40 ng gâ1 produced determination coefficients (R2) above 0.997 for all matrices.