A novel enzymatic approach based on the use of multi-enzymatic systems for the recovery of enriched protein extracts from potato pulp

•Multi-enzymatic systems were investigated for their potential to isolate non-denatured potato proteins.•Multi-enzymatic systems with the same enzyme activity profiles led to similar protein recovery yields.•DEP and CER extracts were enriched with patatin and protease inhibitors, respectively.•Efficiency of DEP-based treatment was dependent on the interactive effect of enzymatic units and time.•Efficiency of CER-based treatment was affected by the quadratic effect of enzymatic units and time.

Ten commercial available multi-enzymatic systems have been explored for the efficient recovery of patatin and protease inhibitors from potato pulp. Their enzyme activity profile was characterized, where corresponding enzyme activity profile led to similar protein recovery yield. Of those assessed, Depol 670L (DEP) and Ceremix 2XL (CER) were efficient for the recovery of protein extract enriched with patatin (up to 60.0%) and protease inhibitors (up to 72.0%), respectively. The efficiency of DEP was significantly dependent on the interactive effect of enzymatic units and incubation time (p-value <0.015), while CER was found to be affected by the quadratric effect of both variables (p-value <0.022-0.052). The enriched patatin DEP-based protein extract possessed higher lipid acyl hydrolase activity emphasizing its preservation. The enriched protease inhibitors CER-based protein extract resulted in higher trypsin inhibiting activity, when compared to the industrial method. DEP and CER-based protein extracts were structurally characterized by peptide mass mapping.