Comparative proteomic analysis of serum of mice infected with Plasmodium berghei ANKA strain using two dimensional gel electrophoresis and matrix-assisted laser desorption ionization - time-of-flight mass spectrometry

•Proteomic analysis of serum of mice infected with Plasmodium berghei.•2D and MALDI analyses show differentially expressed proteins.•Analysis leads to biomarker-based diagnostic tool.

Malaria, worldwide, affects around 500 million people with 2-3 million clinical cases every year. In this study, a proteomic approach was used to analyze the alterations in mice serum proteome induced by P. berghei infection; and identified number of differentially expressed proteins compared to control. Serum proteins were separated by two-dimensional electrophoresis (2DE). These proteins were further identified by matrix-assisted laser desorption or ionization - time-of-flight (MALDI-TOF) spectrometry. Important proteins identified include Serum amyloid A4 protein, Kelch-like protein, Glutathione-S-transferase Mu1. The identified proteins could have a significant role in developing a biomarker-based diagnostic tool for malaria.

Graphical abstractDownload high-res image (119KB)Download full-size image