Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5134839 | Journal of Chromatography A | 2017 | 8 Pages |
â¢Bioanalytical procedure validated in accordance to international guideline.â¢Innovative HILIC-HR/MS approach for detection of in vitro hBCRP ATPase activity.â¢Identification of hBCRP ATPase activators or inhibitors possible.â¢Applied for determination of effects of five HIV protease inhibitors.
Interactions with the human breast cancer resistance protein (hBCRP) significantly influence the pharmacokinetic properties of a drug and can even lead to drug-drug interactions. As efflux pump from the ABC superfamily, hBCRP utilized energy gained by adenosine 5â²-triphosphate (ATP) hydrolysis for the transmembrane movement of its substrates, while adenosine 5â²-diphosphate (ADP) and inorganic phosphate were released. The ADP liberation can be used to detect interactions with the hBCRP ATPase. An ADP quantification method based on hydrophilic interaction liquid chromatography (HILIC) coupled to high resolution tandem mass spectrometry (HR-MS/MS) was developed and successfully validated in accordance to the criteria of the guideline on bioanalytical method validation by the European Medicines Agency. ATP and adenosine 5â²-monophosphate were qualitatively included to prevent interferences. Furthermore, a setup consisting of six sample sets was evolved that allowed detection of hBCRP substrate or inhibitor properties of the test compound. The hBCRP substrate sulfasalazine and the hBCRP inhibitor orthovanadate were used as controls. To prove the applicability of the procedure, the effect of amprenavir, indinavir, nelfinavir, ritonavir, and saquinavir on the hBCRP ATPase activity was tested. Nelfinavir, ritonavir, and saquinavir were identified as hBCRP ATPase inhibitors and none of the five HIV protease inhibitors turned out to be an hBCRP substrate. These findings were in line with a pervious publication.