Functionalized polymer monoliths with carbamylated amylose for the enantioselective reversed phase nano-liquid chromatographic separation of a set of racemic pharmaceuticals

•The first encapsulation of three carbamylated amylose in organic polymer monolith.•Baseline separation was achieved under rare reversed phase chromatographic conditions.•The developed columns provide more economical analysis under environmentally benign conditions.•Chiral recognition mechanism is speculated.

Here we report the first encapsulation of three carbamylated amylose namely R-, S- and R/S-amylose 2,3(3,5-dimethylphenylcarbamate)-6-ethylphenylcarbamate in organic polymer monolith in situ capillary columns. The columns were investigated for the enantioselective nano-liquid chromatographic separation of a set of racemic pharmaceuticals, namely, α- and β-blockers, anti-inflammatory drugs, antifungal drugs, norepinephrine-dopamine reuptake inhibitors, catecholamines, sedative hypnotics, antihistaminics, anticancer drugs, and antiarrhythmic drugs. Baseline separation was achieved for several drugs under reversed phase chromatographic conditions and only few drugs were separated under normal phase conditions. The developed columns provide more economical analysis under environmentally benign conditions.