Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5135294 | Journal of Chromatography A | 2017 | 8 Pages |
â¢The silica nanoparticle-graphene oxide composite (GO@MPBA) was obtained via thiol-yne click (TYC) reaction.â¢The structure and affinity properties of GO@MPBA were characterized.â¢GO@MPBA exhibited the high binding capacity and selectivity towards glycoproteins.â¢GO@MPBA showed good reusability as protein adsorbent.â¢Enrichment of glycoproteins from the biological samples.
In this work, a facile novel strategy is developed for the preparation of 4-mercapto-phenylboronic acid functionalized silica nanoparticle-graphene oxide composite (GO@MPBA) via thiol-yne click reaction. The morphology, structure and composition of the resulting GO@MPBA was characterized by transmission electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, Fourier transform Raman spectroscopy, Thermogravimetric analysis and X-ray photoelectron spectrometry. GO@MPBA exhibited the high binding capacity towards glycoproteins such as ovalbumin (1288.8 mg gâ1), immunoglobulin G (1144.1 mg gâ1), transferrin (592.1 mg gâ1) and horseradish peroxidase (392.4 mg gâ1), in contrast, the binding capacity of each non-glycoproteins (cytochrome c, deoxyribonuclease A, pepsin, trypsin, lysozyme and bovine serum albumin) is less than 50 mg gâ1. Furthermore, the GO@MPBA still remained a good binding capacity after the five times of adsorption-desorption cycles. The selective enrichment of glycoproteins from real egg white samples by GO@MPBA was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. This work could present a facile novel approach of surface modification to design environmentally friendly and more efficient adsorbents for the isolation and enrichment of glycoprotein from complex biological samples.