Superficially porous particles with 1000 Ã pores for large biomolecule high performance liquid chromatography and polymer size exclusion chromatography

•Separation of biomolecules is enhanced using 1000 Å pores in core-shell particles.•Rapid mass transport in 1000 Å pores provide high resolution for large molecules.•DNA fragments, mAbs, large proteins, and polystyrene separations are demonstrated.•Wide pore core-shells are compared with other particle morphologies and pore sizes.

To facilitate mass transport and column efficiency, solutes must have free access to particle pores to facilitate interactions with the stationary phase. To ensure this feature, particles should be used for HPLC separations which have pores sufficiently large to accommodate the solute without restricted diffusion. This paper describes the design and properties of superficially porous (also called Fused-Core®, core shell or porous shell) particles with very large (1000 Å) pores specifically developed for separating very large biomolecules and polymers. Separations of DNA fragments, monoclonal antibodies, large proteins and large polystyrene standards are used to illustrate the utility of these particles for efficient, high-resolution applications.