Investigation of the temperature dependence of water adsorption on silica-based stationary phases in hydrophilic interaction liquid chromatography

The adsorbed water may fill four to eight percent of the pore volume over the studied temperature range, which approximately corresponds to the equivalent of 0.24-0.68 water layer coverage of the adsorbent surface. The phenyl hydride stationary phase shows decreased water uptake in comparison to the Silica C stationary phase.