Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5135562 | Journal of Chromatography A | 2017 | 8 Pages |
Abstract
An analytical method based on pressurised liquid extraction (PLE) followed by liquid chromatography-high resolution mass spectrometry (Orbitrap) was developed for the simultaneous determination of ten high-intensity sweeteners in fish samples. As the method was developed, the different PLE parameters were optimised and different clean-up strategies were evaluated, of which in-cell clean-up using alumina and on-cell clean-up with hexane were the most effective. PLE recoveries were between 43% and 94%. The method quantification limits were between 12.5 ng gâ1 dry weight (d.w.) and 250 ng gâ1 (d.w.) and the method detection limits between 2.5 ng gâ1 (d.w.) and 125 ng gâ1 (d.w.). Intra-day precision and inter-day precision were below 16% and 25%, respectively. Fish samples from different species were analysed and, saccharin was found below its method quantification limit in the species Scomber scombrus (Atlantic mackerel).
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mireia Núñez, Francesc Borrull, Eva Pocurull, Núria Fontanals,