Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5136096 | Journal of Chromatography B | 2017 | 5 Pages |
Abstract
Wildlife contraceptives are an emerging tool for minimizing human-wildlife conflicts. One promising avian contraceptive compound, 20,25-diazacholesterol (DAC), reduces fertility by inhibiting cholesterol synthesis. A reliable analytical method for DAC was required in support of its registration for use as a reproductive control agent in pest bird species. A liquid chromatographic method employing tandem mass spectrometry (LC-MS/MS) was developed for the analysis of tissue extracts following solid phase extraction clean-up. Tissues analyzed were whole body samples from crows, monk parakeets, and quails and liver samples from crows and quails. Excellent sensitivity and selectivity was afforded by tandem mass spectrometry. The method accuracy of DAC from various tissue samples fortified at parts-per-million (ppm) and parts-per-billion (ppb) concentrations was high (>90%) with excellent precision (<10% relative standard deviation). Lower limits of detection were excellent in all tissues types, ranging from 1 to 11Â ppb in whole body matrices and 9.9-34Â ppb in liver matrices.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Benjamin G. Abbo, Laura E. Hulslander, David A. Goldade,