Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5136197 | Journal of Chromatography B | 2017 | 8 Pages |
Abstract
A high-salt, hydrophobic interaction chromatography (HIC) method was developed to measure the relative hydrophobicity of a diverse set of solutes. Through the careful control of buffer pH and salt concentration, this assay was then used to ascertain for the first time the relative hydrophobicity values of three different bacteriophage, four mammalian viruses, and a range of biotech medicinal proteins as benchmarked to protein standards previously characterized for hydrophobicity.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sarah A. Johnson, Alison Walsh, Matthew R. Brown, Scott C. Lute, David J. Roush, Michael S. Burnham, Kurt A. Brorson,