Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5136435 | Journal of Chromatography B | 2017 | 9 Pages |
Abstract
A stable method, using isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS), to simultaneously determine six aldehyde-DNA adducts was developed and applied to the analysis of human salivary DNA samples. The detection limit of these six DNA adducts was in the range of 0.006-0.014 ng/mL and that of the quantification limit was 0.017-0.026 ng/mL. The intra-day and inter-day precision of all aldehyde-DNA adducts was <10%. The analysis was completed within 25 min. Additionally, a noninvasive technique was used to collect the DNA samples from human saliva. The new method was successfully applied for the analysis of salivary DNA of nonsmokers and smokers. Five aldehyde-DNA adducts were detected in both smoker and nonsmoker salivary DNA, while α-Acr-dG was not detected in all the samples. Among these detected DNA adducts, no significant differences were found between smoker and nonsmoker (p > 0.05). This may due to the individual detoxifying differences or environmental and endogenous exposure. Our study provides a rapid and selective method to simultaneously detect six aldehyde-DNA adducts and to assess potential DNA damage induced by aldehydes,
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xiangyu Li, Lujuan Liu, Hongjuan Wang, Jian Chen, Beibei Zhu, Huan Chen, Hongwei Hou, Qingyuan Hu,