Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5136502 | Journal of Chromatography B | 2017 | 10 Pages |
â¢Report of non-fatal and overdose case involving 25B-NBOMe and 4-CMC.â¢Quantification of 25B-NBOMe and 4-CMC in blood by UPLC-MS/MS.â¢Clinical symptoms during intoxication.
This paper describes cases of poisoning caused by new psychoactive substances such as: 25B-NBOMe (2-(4-bromo-2,5-dimethoxyphenyl)-N-[(2-methoxyphenyl)methyl]ethanamine) and 4âCMC (1-(4-chlorophenyl)-2-(methylamino)-1-propanone). The analytical procedure includes rapid and selective method for the extraction and determination of 4-CMC and 25B-NBOMe in blood samples using UPLC-MS/MS technique. To the best of our knowledge, this is the first report, that involves a fully validated method for quantification of new-designer drug - 4-CMC in postmortem blood samples. The biological material was also analyzed with the use of routine analytical methods: immunochemical techniques, gas chromatography with flame ionization detection and gas chromatography with electron impact mass spectrometry. The results of real samples analyses correspond to possible toxicological effects: death resulting from 25B-NBOMe - mediated hallucinations (661Â ng/mL of 25BâNBOMe and 0.887Â ng/mL of 4-CMC), fatal overdose of 25B-NBOMe and 4-CMC (66.5Â ng/mL of 25BâNBOMe and 2.14Â ng/mL of 4-CMC) and non-fatal intoxication of these drugs (38.4Â ng/mL of 25B NBOMe and 0.181Â ng/mL of 4-CMC). Additionally, O-demethylathed O, O-bis-demethylathed and glucuronidated metabolites of 25B-NBOMe in biological specimens were detected.