Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5136731 | Journal of Chromatography B | 2016 | 12 Pages |
Abstract
Antibody-drug conjugates might be the magic bullets referred to by Paul Ehrlich over 100 years ago. Together with a huge therapeutic potential, these molecules come with a structural complexity that drives state-of-the-art chromatography and mass spectrometry to its limits. The use of multiple heart-cutting (mLC-LC) and comprehensive (LCÂ ÃÂ LC) multidimensional LC in combination with high resolution mass spectrometry for the characterization of the lysine conjugated antibody-drug conjugate ado-trastuzumab emtansine, commercialized as Kadcyla, is presented. By combining protein and peptide measurements, attributes such as drug loading, drug distribution and drug conjugation sites can be assessed in an elegant manner.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Koen Sandra, Gerd Vanhoenacker, Isabel Vandenheede, Mieke Steenbeke, Maureen Joseph, Pat Sandra,