Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5136773 | Journal of Chromatography B | 2017 | 25 Pages |
Abstract
The development and validation of an ultra-high pressure liquid chromatography (UHPLC) tandem mass spectrometry (MS/MS) method was performed with the aim to be applied for the quantification of plasma teicoplanin concentrations in neonates. Pharmacokinetic data of teicoplanin in the neonatal population is very limited, therefore, a sensitive and reliable method for the determination of all isoforms of teicoplanin applied in a low volume of sample is of real importance. Teicoplanin main components were extracted by a simple acetonitrile precipitation step and analysed on a C18 chromatographic column by a triple quadrupole MS with electrospray ionization. The method provides quantitative data over a linear range of 25-6400Â ng/mL with LOD 8.5Â ng/mL and LOQ 25Â ng/mL for total teicoplanin. The method was applied in plasma samples from neonates to support pharmacokinetic data and proved to be a reliable and fast method for the quantification of teicoplanin concentration levels in plasma of infants during therapy in Intensive Care Unit.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
O. Begou, A. Kontou, N. Raikos, K. Sarafidis, E. Roilides, I.N. Papadoyannis, H.G. Gika,