Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5137843 | Journal of Pharmaceutical and Biomedical Analysis | 2017 | 11 Pages |
Abstract
The analytical response was linear (r2 â¥Â 0.99) in the concentration (ng/mL) ranges: 50-10 000 for FβAL and FUH2, 50-5 000 for FUPA, 50-100 000 for 5-FU, 5-200 for U and 10-400 for UH2. Within- and between-run accuracy and precision were ⤠10.2% and ⤠9.8% respectively across the QC range and inclusive of LLOQ. The internal standard (IS) normalised matrix effects were within 93-112% with CV of ⤠9.7% and normalised recoveries were within 91-107% with CV of ⤠9.8%. This robust assay was successfully applied to samples from rectal and colorectal cancer patients (n = 10) on 5-FU. Deviations ⤠2.0% from the mean values were observed when incurred samples were reanalysed.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ottiniel Chavani, Berit Packert Jensen, R. Matthew Strother, Christopher M. Florkowski, Peter M. George,