Capillary electrophoresis in the context of drug discovery

Article ID	Journal	Published Year	Pages	File Type
5137950	Journal of Pharmaceutical and Biomedical Analysis	2017	58 Pages	PDF

Abstract

The objective of this review is to focus our attention on recent developments of this technique in three different drug discovery areas: bioassays, drug-plasma interactions and drug metabolism studies. These developments were based on two important abilities of CE: the capacity to measure non-covalent interactions in solution and the ability to use a portion of the capillary as a reactor while the rest of the capillary is used for the separation of the product of the reaction.

Keywords

FAM HEPES HDL GFP IC50 NADPH PPI ACE ITC EOF EMMA DLLME qTOF NCE hsp70 Vmax ppb AGP HLM HNE HDM α-acid glycoprotein EMEA VLDL CYP450 FXa FIIa β2-AR FMOs DMEs MOPS DDIs BGE IMER FBDD BAG3 NECEEM O-desmethylvenlafaxine FDA HPAC LIF 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid DMSO DNA Dispersive liquid–liquid microextraction Bioassays amlodipine antithrombin III drug metabolizing enzymes European Medicines agency Plasma protein binding internal standard deoxyribonucleic acid affinity capillary electrophoresis vacancy affinity capillary electrophoresis Capillary electrophoresis background electrolyte Transverse diffusion of laminar flow profiles Human neutrophil elastase Ultrafiltration Fourier transform infrared Frontal analysis Electrophoretic Mobility Tris(hydroxymethyl)aminomethane hydrochloride Tris-HCl Activity assays Protein–protein interaction Drug–drug interactions Inhibitory constant Electroosmotic flow Dissociation constant maximum velocity Michaelis-Menten constant equilibrium dialysis Dimethyl sulfoxide Immobilized enzyme reactor quadrupole time-of-flight Food and Drug Administration Cytochrome P450 FTIR pharmacokinetic Laser induced fluorescence LDL High-density lipoproteins very-low-density lipoproteins low-density lipoproteins Drug metabolism Ver new chemical entity Electrophoretically mediated microanalysis Human Liver Microsomes half maximal inhibitory concentration nicotinamide adenine dinucleotide phosphate reduced Affinity constant polymerase chain reaction PCR Verapamil Venlafaxine heat shock protein 70 Green fluorescence protein Propranolol hydrochloride polyethylene glycol PEG chylomicrons Isothermal titration calorimetry carboxyfluorescein High performance affinity chromatography Fragment based drug discovery β2-adrenergic receptor