Stable isotope labeling by fatty acids in cell culture (SILFAC) coupled with isotope pattern dependent mass spectrometry for global screening of lipid hydroperoxide-mediated protein modifications

Article ID	Journal	Published Year	Pages	File Type
5138450	Journal of Proteomics	2017	58 Pages	PDF

Keywords

HSA ASCA DGLA IAA FDR THR ASN pTH DNPH DTT GLN phenylthiohydantoin MCF-7 DPBS HNE GRP DDS GLA BCA Cys ICAT PLPC SRM PTM IDDS CID PlpE ESI Total Ion Current Chromatogram 13-HPODE 4-hydroxy-2(E)-nonenal HPNE FBS DMEM 2,4-dinitrophenyl hydrazine 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide BSA Dulbecco's modified Eagle's medium MS/MS MTT ROS Arginine Asparagine Ascorbic acid human serum albumin bovine serum albumin EDTA Ethylenediaminetetraacetic acid Collision-induced dissociation Arg internal standard Arachidonic acid Linoleic acid Polyunsaturated fatty acid PUFA post-translational modification Protein modification collision energy SER isobaric tag for relative and absolute quantitation bicinchoninic acid threonine Oxidative stress Isotope-coded affinity tags Tyr Tyrosine Human breast adenocarcinoma cell line standard error of the mean HIS dithiothreitol fetal bovine serum Serine Cysteine SILAC Mass spectrometry Tandem mass spectrometry Dulbecco's phosphate buffered saline phosphatidylcholine phosphatidylethanolamine Lysine LYS Methionine SEM MET false discovery rate stable isotope labeling by amino acids in cell culture selected reaction monitoring histidine Lipid peroxidation liquid chromatography glutamine Reactive oxygen species electrospray ionization iodoacetamide ONE

Related Topics

Physical Sciences and Engineering Chemistry Analytical Chemistry

Stable isotope labeling by fatty acids in cell culture (SILFAC) coupled with isotope pattern dependent mass spectrometry for global screening of lipid hydroperoxide-mediated protein modifications

Authors

Ryo Takahashi, Shuhei Fujioka, Tomoyuki Oe, Seon Hwa Lee,