Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5139003 | Microchemical Journal | 2017 | 9 Pages |
Abstract
The electrochemical behaviour of the antitumor drug mitomycin C (MMC) was investigated on carbon paste electrode over a wide pH range and using voltammetric techniques. The MMC undergoes diffusion-controlled irreversible oxidation in two different processes, one pH-independent for 2.2Â ËÂ pHÂ ËÂ 4.5 and one pH-dependent for 4.5Â ËÂ pHÂ ËÂ 12.0, and does not involve the formation of any electroactive oxidation product. Upon incubation in different pH electrolytes, chemical degradation of MMC was electrochemically detected by the appearance of a new oxidation peak at a lower potential. The chemically degraded MMC undergoes an irreversible, pH-dependent oxidation for 3.4Â ËÂ pHÂ ËÂ 5.4, and its redox products are reversibly oxidised. The spontaneous degradation of MMC in aqueous solution was confirmed by UV-Vis spectrophotometry. Moreover, a multilayer dsDNA-electrochemical biosensor was used to evaluate the interaction between MMC and DNA. The results have clearly proven that MMC interacts and binds to dsDNA strands immobilized onto a glassy carbon electrode surface and its metabolite(s) cause oxidative damage to DNA.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Evellin Enny S. Bruzaca, Ilanna C. Lopes, Elizaura Hyeda C. Silva, Paulina Andréa V. Carvalho, Auro A. Tanaka,