Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5139039 | Microchemical Journal | 2017 | 40 Pages |
Abstract
The photochemical behavior of folic acid and its metabolites, tetrahydrofolic and 5-methyltetrahydrofolic acids has been followed by high performance liquid chromatography, coupled with a fast scanning fluorimetric detector (HPLC-FSFD). The main photoproducts generated were characterized through accurate mass measurement using liquid chromatography-mass spectrometry (HPLC-MS). The effect on photogeneration processes of irradiation time, and the hydrogen peroxide concentration, in presence and in absence of N2 stream, was also studied. A chromatographic method with post-column on-line photoderivatization has been developed for the analysis of folic, tetrahydrofolic and 5-methyltetrahydrofolic acids. Linearity ranges from 50 to 1000 ng·mLâ 1 for folic acid, and from 100 to 1000 ng·mLâ 1 for tetrahydrofolic and 5-methyltetrahydrofolic acids have been established. Detection limits of 7.5 ng·mLâ 1, 15.3 ng·mLâ 1 and 22.9 ng·mLâ 1 were calculated for folic acid, tetrahydrofolic acid and 5-methyltetrahydrofolic acid respectively. The method has been satisfactorily applied to cereals, spinach and tomato analysis. Serum samples analysis was also tested.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
ElÃsabet MartÃn Tornero, Anunciación Espinosa-Mansilla, Isabel Durán Merás,